Proyield® Soy SE50MAF-UF is an animal component-free protein hydrolysate manufactured by FrieslandCampina Ingredients. It is widely used in mammalian cell culture processes, such as Chinese hamster ovary (CHO) cell systems, as part of basal or feed media formulation. It has been shown to complement commercially available chemically defined (CD) media to enhance cell culture performance. The manufacturing of Proyield® Soy SE50MAF-UF involves multiple steps including protein hydrolysis, multiple filtration steps and drying of the end product to powder. In this poster, FrieslandCampina Ingredients presents the work from our Quality by Design (QbD) project to demonstrate the use of production data and statistical analysis. The goal is to elucidate how the chemical composition of the hydrolysate can be controlled via process.
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